Incorporation was inhibited by 75% in the presence of 1 mM exogenous DHA and by 50% in the presence of 1 mM exogenous linoleic acid. When incubated in human plasma, macrophages incorporated 20% of the incorporated DHA into phospholipids and 80% into triacylglycerols. The bioactivity of the medium-derived DHA in macrophages was determined by measuring prostaglandin E(2) (PGE(2)) release. Macrophages were incubated in control medium, human plasma, or dexamethasone (DEX) medium for 4 h, and DHA incorporation and catabolism were examined by gas chromatography mass spectrometry. The objective of this study was to determine the effect of glucocorticoids on the biosynthesis, catabolism, and turnover of docosahexaenoate (22:6n-3, DHA) in human monocyte-derived macrophages. This Autodesk Revit 2021 License Key is.Glucocorticoids and the metabolic fate of docosahexaenoate in human monocyte-derived macrophages.
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